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Titel: Microsatellites from Fosterella christophii (Bromeliaceae) by de novo transcriptome sequencing on the Pacific Biosciences RS platform
Autor(en): Wöhrmann, TinaHuettel, BrunoWagner, NataschaWeising, Kurt
Klassifikation (DDC): 580 - Pflanzen (Botanik) (Botanical sciences)
Erscheinungsdatum: 2016
Zitierform: In: Applications in plant sciences. - Washington, DC : BioOne, 2016, 4 (1):1500084, 1-4
Zusammenfassung: • Premise of the study: Microsatellite markers were developed in Fosterella christophii (Bromeliaceae) to investigate the genetic diversity and population structure within the F. micrantha group, comprising F. christophii, F. micrantha, and F. villosula. • Methods and Results: Full-length cDNAs were isolated from F. christophii and sequenced on a Pacific Biosciences RS platform. A total of 1590 high-quality consensus isoforms were assembled into 971 unigenes containing 421 perfect microsatellites. Thirty primer sets were designed, of which 13 revealed a high level of polymorphism in three populations of F. christophii, with four to nine alleles per locus. Each of these 13 loci cross-amplified in the closely related species F. micrantha and F. villosula, with one to six and one to 11 alleles per locus, respectively. • Conclusions: The new markers are promising tools to study the population genetics of F. christophii and to discover species boundaries within the F. micrantha group.
URI: urn:nbn:de:hebis:34-2016020549834
zus. URI: doi:10.3732/apps.1500084OA-GEF
ISSN: 2168-0450
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