Dissertation
Artificial micro RNA system in Dictyostelium discoideum
Zusammenfassung
Dictyostelium discoideum is a social amoeba that serves as a model system for RNA interference and related mechanisms. Its position between plants and animals enables evolutionary snapshot of mechanisms and protein machinery involved in investigated subjects. MiRNAs are small regulatory RNAs that are evolutionary conserved and present in animals, plants, viruses and some prokaryotes. They have roles in development, cell growth and differentiation, apoptosis and their miss-regulation is associated with many diseases such as cancer, neurodegenerative disorders and diabetes. Recently, through sequencing of DNA libraries miRNAs have been discovered in D. discoideum. In this work, it has been shown that heterologues miRNA let-7 can be expressed and processed in D. discoideum. Expression of let-7 miRNA in social amoeba resulted in a strong developmental phenotype suggesting an overload of the processing/silencing system or/and endogenous targets. The various effects on prel-7 strain have been observed and characterized, serving as a background for postulation of miRNA roles. An artificial miRNA system has been established and imposed to D. discoideum, showing that miRNAs in Dictyostelium could mediate gene expression on the level of mRNA stability and on the posttranscriptional level. Furthermore, presence of translational inhibition as a type of gene control was shown for the first time in this organism. Due to it new structures representing co-localities of miRNA and target mRNA have been detected. Taken together, this work shows functional artificial miRNA system and postulates roles of endogenous small RNA in social amoeba.
Zitieren
@phdthesis{urn:nbn:de:hebis:34-2011011235316,
author={Andonovic, Lidija},
title={Artificial micro RNA system in Dictyostelium discoideum},
school={Kassel, Universität, FB 10, Mathematik und Naturwissenschaften, Institut für Biologie},
month={01},
year={2011}
}
0500 Oax 0501 Text $btxt$2rdacontent 0502 Computermedien $bc$2rdacarrier 1100 2011$n2011 1500 1/eng 2050 ##0##urn:nbn:de:hebis:34-2011011235316 3000 Andonovic, Lidija 4000 Artificial micro RNA system in Dictyostelium discoideum / Andonovic, Lidija 4030 4060 Online-Ressource 4085 ##0##=u http://nbn-resolving.de/urn:nbn:de:hebis:34-2011011235316=x R 4204 \$dDissertation 4170 5550 {{Dictyostelium discoideum}} 5550 {{RNS}} 7136 ##0##urn:nbn:de:hebis:34-2011011235316
2011-01-12T12:10:45Z 2011-01-12T12:10:45Z 2011-01-12T12:10:45Z urn:nbn:de:hebis:34-2011011235316 http://hdl.handle.net/123456789/2011011235316 eng Urheberrechtlich geschützt https://rightsstatements.org/page/InC/1.0/ micro RNA Dictystelium discoideum small RNA 570 Artificial micro RNA system in Dictyostelium discoideum Dissertation Dictyostelium discoideum is a social amoeba that serves as a model system for RNA interference and related mechanisms. Its position between plants and animals enables evolutionary snapshot of mechanisms and protein machinery involved in investigated subjects. MiRNAs are small regulatory RNAs that are evolutionary conserved and present in animals, plants, viruses and some prokaryotes. They have roles in development, cell growth and differentiation, apoptosis and their miss-regulation is associated with many diseases such as cancer, neurodegenerative disorders and diabetes. Recently, through sequencing of DNA libraries miRNAs have been discovered in D. discoideum. In this work, it has been shown that heterologues miRNA let-7 can be expressed and processed in D. discoideum. Expression of let-7 miRNA in social amoeba resulted in a strong developmental phenotype suggesting an overload of the processing/silencing system or/and endogenous targets. The various effects on prel-7 strain have been observed and characterized, serving as a background for postulation of miRNA roles. An artificial miRNA system has been established and imposed to D. discoideum, showing that miRNAs in Dictyostelium could mediate gene expression on the level of mRNA stability and on the posttranscriptional level. Furthermore, presence of translational inhibition as a type of gene control was shown for the first time in this organism. Due to it new structures representing co-localities of miRNA and target mRNA have been detected. Taken together, this work shows functional artificial miRNA system and postulates roles of endogenous small RNA in social amoeba. open access Andonovic, Lidija Kassel, Universität, FB 10, Mathematik und Naturwissenschaften, Institut für Biologie Nellen, Wolfgang (Prof. Dr.) Maniak, Markus (Prof. Dr.) Dictyostelium discoideum RNS 2010-06-23
Die folgenden Lizenzbestimmungen sind mit dieser Ressource verbunden:
:Urheberrechtlich geschützt