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Pollen metabarcoding of museum specimens and recently collected bumblebees (Bombus) indicates foraging shifts

dc.date.accessioned2023-07-21T11:22:31Z
dc.date.available2023-07-21T11:22:31Z
dc.date.issued2023-07-05
dc.identifierdoi:10.17170/kobra-202307218435
dc.identifier.urihttp://hdl.handle.net/123456789/14907
dc.description.sponsorshipGefördert durch den Publikationsfonds der Universität Kassel
dc.language.isoeng
dc.rightsNamensnennung 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectbarcodingeng
dc.subjectBombuseng
dc.subjectbumblebeeeng
dc.subjectCuxhaveneng
dc.subjectHamburgeng
dc.subjectITS1eng
dc.subjectITSeng
dc.subjectITS2eng
dc.subjectnatural history collectioneng
dc.subjectplant metabarcodingeng
dc.subjectpolleneng
dc.subjecttrnL-F P6engeng
dc.subject.ddc570
dc.subject.ddc580
dc.subject.ddc590
dc.titlePollen metabarcoding of museum specimens and recently collected bumblebees (Bombus) indicates foraging shiftseng
dc.typeAufsatz
dcterms.abstractLandscape changes, over time, lead to changes of floral resources available to pollinators, which in turn may result in the disappearance of ecologically specialized species. Here, we use pollen metabarcoding to infer historic and recent interactions between plants and bumblebees (Bombus). Bumblebees from Cuxhaven (Germany) were sampled from historical museum collections (1968/69) and in the field (2019). Pollen attached to their bodies was barcoded using multiple plant markers (ITS1, ITS2 and trnL-P6 loop). Our results show shifts in foraging habits between the historic and recent sampling periods, mostly determined by fewer Fabaceae interactions in 2019. The successful implementation of scalable molecular techniques for the analysis of historical pollen samples underscores the value of museum collections as a resource for biodiversity research. This study provides proof of concept of a comparative analysis of recent and historical pollination data. However, to ensure the robustness of our results, it is crucial to consider the broader methodology used. Our study found variation in the efficacy of the three plant barcoding markers. The ITS1 marker exhibited the highest species-level identification success, while the trnL-P6 loop demonstrated utility in amplifying degraded DNA across diverse plant families.eng
dcterms.accessRightsopen access
dcterms.creatorKolter, Andreas
dcterms.creatorHusemann, Martin
dcterms.creatorPodsiadlowski, Lars
dcterms.creatorGemeinholzer, Birgit
dc.relation.doidoi:10.3897/mbmg.7.86883
dc.subject.swdDeutschlandger
dc.subject.swdCuxhavenger
dc.subject.swdHamburgger
dc.subject.swdPollenger
dc.subject.swdDNA Barcodingger
dc.subject.swdHummelger
dc.subject.swdNahrungserwerbger
dc.type.versionpublishedVersion
dcterms.source.identifiereissn:2534-9708
dcterms.source.journalMetabarcoding and Metagenomicseng
dcterms.source.pageinfo89-119
dcterms.source.volume7
kup.iskupfalse
dcterms.source.articlenumbere86883


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